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isotype matched control antibody  (R&D Systems)


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    Structured Review

    R&D Systems isotype matched control antibody
    Isotype Matched Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isotype+matched+control+antibody/Mouse+IgG2B+APC-conjugated+Antibody/us12613244-531-21-26
    Average 93 stars, based on 103 article reviews
    isotype matched control antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunodepletion:

    Article Title: Rad50-CARD9 interactions link cytosolic DNA sensing to IL-1β production.
    Article Snippet: .. For the immunodepletion of Rad50, THP-1 lysates were incubated for up to three consecutive rounds with anti-Rad50 (sc-56209; Santa Cruz) or isotype-matched control antibody (Mouse IgG1 Isotype Control; MAB002; R&D Systems) immobilized on Sepharose beads. ..

    Incubation:

    Article Title: Rad50-CARD9 interactions link cytosolic DNA sensing to IL-1β production.
    Article Snippet: .. For the immunodepletion of Rad50, THP-1 lysates were incubated for up to three consecutive rounds with anti-Rad50 (sc-56209; Santa Cruz) or isotype-matched control antibody (Mouse IgG1 Isotype Control; MAB002; R&D Systems) immobilized on Sepharose beads. ..

    Article Title: Method for rapid accurate dispensing, visualization and analysis of single cells
    Article Snippet: One-half of the volume of the above suspension was incubated with 10 uL of an appropriate, conjugated monoclonal antibody (mouse anti-human ERBB2 (HER2)-APC; R&D Systems; FAB1129A) against the cell surface antigen ERBB2 (HER2/neu). .. The remaining half-volume of the suspension was incubated in parallel with an appropriate negative control antibody for immunostaining, namely, a conjugated, isotype-matched control antibody (mouse IgG2B-APC; R&D Systems; IC0041A). ..

    Article Title: NZ28-induced inhibition of HSF1, SP1 and NF-κB triggers the loss of the natural killer cell-activating ligands MICA/B on human tumor cells.
    Article Snippet: .. Flow cytometry Cells were incubated with the APC-conjugated MICA (clone 159227, FAB1300A, R&D Systems) and MICB antibodies (clone 236511, FAB1599A, R&D Systems) or the corresponding isotype-matched control antibody (clone 133303, IC0041A, R&D Systems) for 30 min at 4 °C. .. Dead cells were stained with propidium iodide, and only viable cells were analyzed on a FACSCalibur flow cytometer (BD Biosciences).

    Control:

    Article Title: Rad50-CARD9 interactions link cytosolic DNA sensing to IL-1β production.
    Article Snippet: .. For the immunodepletion of Rad50, THP-1 lysates were incubated for up to three consecutive rounds with anti-Rad50 (sc-56209; Santa Cruz) or isotype-matched control antibody (Mouse IgG1 Isotype Control; MAB002; R&D Systems) immobilized on Sepharose beads. ..

    Article Title: Method for rapid accurate dispensing, visualization and analysis of single cells
    Article Snippet: One-half of the volume of the above suspension was incubated with 10 uL of an appropriate, conjugated monoclonal antibody (mouse anti-human ERBB2 (HER2)-APC; R&D Systems; FAB1129A) against the cell surface antigen ERBB2 (HER2/neu). .. The remaining half-volume of the suspension was incubated in parallel with an appropriate negative control antibody for immunostaining, namely, a conjugated, isotype-matched control antibody (mouse IgG2B-APC; R&D Systems; IC0041A). ..

    Article Title: Triggering receptor expressed on myeloid cells-1 (TREM-1) amplifies the signals induced by the NACHT-LRR (NLR) pattern recognition receptors.
    Article Snippet: .. To activate PBMC through TREM-1, 96-well flat-bottom plates were precoated with 10 g/ml (unless otherwise indicated) of an agonist anti-TREM-1 mAb (Clone 193015, R&D Systems, Minneapolis, MN) or an isotype-matched control antibody (mouse IgG1, R&D Systems), as previously used also by others [10]. ..

    Article Title: NZ28-induced inhibition of HSF1, SP1 and NF-κB triggers the loss of the natural killer cell-activating ligands MICA/B on human tumor cells.
    Article Snippet: .. Flow cytometry Cells were incubated with the APC-conjugated MICA (clone 159227, FAB1300A, R&D Systems) and MICB antibodies (clone 236511, FAB1599A, R&D Systems) or the corresponding isotype-matched control antibody (clone 133303, IC0041A, R&D Systems) for 30 min at 4 °C. .. Dead cells were stained with propidium iodide, and only viable cells were analyzed on a FACSCalibur flow cytometer (BD Biosciences).

    Article Title: Role of JAK-STAT signaling in the pathogenic behavior of fibroblast-like synoviocytes in rheumatoid arthritis: Effect of the novel JAK inhibitor peficitinib.
    Article Snippet: .. IL6 receptor-blocking antibody (MAB227) and isotype-matched control antibody (MAB002) were purchased from R&D Systems (Minneapolis, MN) and diluted in culture medium. ..

    Article Title: Methods to diagnose and treat attention-deficit, hyperactivity disorder (ADHD)
    Article Snippet: .. Cells were fixed with paraformaldehyde and stained with specific mGluR5 monoclonal antibody (R&D, Cat# MAB4514) or isotype matched control antibody (R&D, Cat# MAB002) followed by phycoerythrin-conjugated anti-mouse antibody. .. Stained cells were analyzed by using BD FACS Calibur flow cytometer.

    Article Title: Goblet Cell Derived RELM-β Recruits CD4 + T Cells during Infectious Colitis to Promote Protective Intestinal Epithelial Cell Proliferation
    Article Snippet: After removal of secondary antibodies, cells were washed 3X with PBS followed by mounting in Vectashield (Vector Laboratories, Burlington, ON, Canada) on glass slides and screened with a Zeiss microscope. .. IL-22 neutralization studies were carried out in C . rodentium infected C57BL/6 mice by injecting them with either neutralizing IL-22 antibodies (R&D systems), PBS or with an isotype matched control antibody (R&D systems) given on 0 and 6 DPI (100μg/mouse). ..

    Article Title: Variation in macrophage-migration-inhibitory-factor immunoreactivity during porcine gestation.
    Article Snippet: The sections were then washed for 5 min in running tap water and mounted with aqueous mountant Aquatex (Merck, Darmstadt, Germany). .. Negative controls were performed for each tissue by substituting the primary antibody with an isotype matched control antibody (R&D Systems) or TBS. ..

    Suspension:

    Article Title: Method for rapid accurate dispensing, visualization and analysis of single cells
    Article Snippet: One-half of the volume of the above suspension was incubated with 10 uL of an appropriate, conjugated monoclonal antibody (mouse anti-human ERBB2 (HER2)-APC; R&D Systems; FAB1129A) against the cell surface antigen ERBB2 (HER2/neu). .. The remaining half-volume of the suspension was incubated in parallel with an appropriate negative control antibody for immunostaining, namely, a conjugated, isotype-matched control antibody (mouse IgG2B-APC; R&D Systems; IC0041A). ..

    Negative Control:

    Article Title: Method for rapid accurate dispensing, visualization and analysis of single cells
    Article Snippet: One-half of the volume of the above suspension was incubated with 10 uL of an appropriate, conjugated monoclonal antibody (mouse anti-human ERBB2 (HER2)-APC; R&D Systems; FAB1129A) against the cell surface antigen ERBB2 (HER2/neu). .. The remaining half-volume of the suspension was incubated in parallel with an appropriate negative control antibody for immunostaining, namely, a conjugated, isotype-matched control antibody (mouse IgG2B-APC; R&D Systems; IC0041A). ..

    Immunostaining:

    Article Title: Method for rapid accurate dispensing, visualization and analysis of single cells
    Article Snippet: One-half of the volume of the above suspension was incubated with 10 uL of an appropriate, conjugated monoclonal antibody (mouse anti-human ERBB2 (HER2)-APC; R&D Systems; FAB1129A) against the cell surface antigen ERBB2 (HER2/neu). .. The remaining half-volume of the suspension was incubated in parallel with an appropriate negative control antibody for immunostaining, namely, a conjugated, isotype-matched control antibody (mouse IgG2B-APC; R&D Systems; IC0041A). ..

    Flow Cytometry:

    Article Title: NZ28-induced inhibition of HSF1, SP1 and NF-κB triggers the loss of the natural killer cell-activating ligands MICA/B on human tumor cells.
    Article Snippet: .. Flow cytometry Cells were incubated with the APC-conjugated MICA (clone 159227, FAB1300A, R&D Systems) and MICB antibodies (clone 236511, FAB1599A, R&D Systems) or the corresponding isotype-matched control antibody (clone 133303, IC0041A, R&D Systems) for 30 min at 4 °C. .. Dead cells were stained with propidium iodide, and only viable cells were analyzed on a FACSCalibur flow cytometer (BD Biosciences).

    Staining:

    Article Title: Methods to diagnose and treat attention-deficit, hyperactivity disorder (ADHD)
    Article Snippet: .. Cells were fixed with paraformaldehyde and stained with specific mGluR5 monoclonal antibody (R&D, Cat# MAB4514) or isotype matched control antibody (R&D, Cat# MAB002) followed by phycoerythrin-conjugated anti-mouse antibody. .. Stained cells were analyzed by using BD FACS Calibur flow cytometer.

    Neutralization:

    Article Title: Goblet Cell Derived RELM-β Recruits CD4 + T Cells during Infectious Colitis to Promote Protective Intestinal Epithelial Cell Proliferation
    Article Snippet: After removal of secondary antibodies, cells were washed 3X with PBS followed by mounting in Vectashield (Vector Laboratories, Burlington, ON, Canada) on glass slides and screened with a Zeiss microscope. .. IL-22 neutralization studies were carried out in C . rodentium infected C57BL/6 mice by injecting them with either neutralizing IL-22 antibodies (R&D systems), PBS or with an isotype matched control antibody (R&D systems) given on 0 and 6 DPI (100μg/mouse). ..

    Infection:

    Article Title: Goblet Cell Derived RELM-β Recruits CD4 + T Cells during Infectious Colitis to Promote Protective Intestinal Epithelial Cell Proliferation
    Article Snippet: After removal of secondary antibodies, cells were washed 3X with PBS followed by mounting in Vectashield (Vector Laboratories, Burlington, ON, Canada) on glass slides and screened with a Zeiss microscope. .. IL-22 neutralization studies were carried out in C . rodentium infected C57BL/6 mice by injecting them with either neutralizing IL-22 antibodies (R&D systems), PBS or with an isotype matched control antibody (R&D systems) given on 0 and 6 DPI (100μg/mouse). ..



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